Effects of functional training on postoperative anterior cruciate injury in athletes hospitalized / Efeitos do treinamento funcional hospitalar no pós-operatório do cruzado anterior em atletas / Efectos del entrenamiento funcional intrahospitalario en la lesión postoperatoria del cruzado anterior en atletas

ABSTRACT Introduction: Anterior cruciate ligament rupture is a common clinical sports injury, and its incidence has an increasing tendency. The understanding by nurses of the best practices in the postoperative period of various surgeries has contributed to the early return of patients to their activities. Therefore, there is an urgent demand for optimizing the hospital protocol for the postoperative stage. Objective: Optimize perioperative nursing care and postoperative functional exercises in sports ligament injuries. Methods: Thirty patients (18 males, age 29.2±8.7 years) with knee cruciate ligament injury admitted to Sanya University Hospital from January to December were selected. Three to five days after admission, based on a regular physical and chemical examination and cruciate ligament reconstruction surgery under epidural anesthesia. Prevention care for postoperative complications, monitoring of vital signs and alternating position on the stretcher, mobilizations, and pain care were detailed. According to the Lysholm and Tegner activity scales, evaluation was made in the functional loss and level of recovery caused by the knee ligament injury. Results: The hospital stay in this group was 12 to 43 days, with a mean of (25.4±8.6) days. After two months of follow-up, 11 cases showed excellent functional recovery, 17 cases of functional recovery were good, the excellent and good index was 93.3%. After six months of follow-up, all patients in this group returned to work and normal life. Conclusion: Optimized treatment with functional training in the post-surgical period, good care, and adapted exercises are of great importance for the brief recovery of a sports function. Evidence Level II; Therapeutic Studies - Investigating the results.

RESUMO Introdução: A ruptura do ligamento cruzado anterior é uma lesão clínica esportiva comum e sua incidência tem uma tendência crescente. A compreensão pelas enfermeiras sobre as melhores práticas no pós-operatório de várias cirurgias tem colaborado para o retorno precoce dos pacientes às suas atividades. Urge a necessidade de uma otimização no protocolo hospitalar para esse pós-cirúrgico. Objetivo: Otimizar as condutas de enfermagem perioperatória e os exercícios funcionais pós-operatórios das lesões ligamentares esportivas. Métodos: Trinta pacientes (18 homens, idade de 29.2±8.7 anos) com lesão do ligamento cruzado do joelho internados no hospital da Universidade Sanya entre janeiro a dezembro foram selecionados. Decorridos 3 a 5 dias após a admissão, com base em exames físicos e químicos regulares, e cirurgia de reconstrução do ligamento cruzado sob anestesia peridural. Foram detalhados os cuidados de prevenção para complicações no pós-operatório, monitoramento de sinais vitais e alternância de posição na maca, mobilizações e cuidados com a dor. A debilidade funcional e o nível de recuperação causados pela lesão ligamentar do joelho foram avaliados de acordo com a escala de pontuação de Lysholm e a escala de atividade de Tegner. Resultados: A permanência hospitalar nesse grupo foi de 12 a 43 dias, com uma média de (25,4±8,6) dias. Após 2 meses de acompanhamento, 11 casos mostraram excelente recuperação funcional, 17 casos de recuperação funcional foram bons, o índice excelente e bom foi de 93,3%. Depois de 6 meses de acompanhamento, todos os pacientes desse grupo voltaram ao trabalho e à vida normal. Conclusão: O tratamento otimizado com treinamento funcional no pós-cirúrgico, bons cuidados e exercícios adaptados são de grande importância para a breve recuperação da função esportiva. Nível de evidência II; Estudos Terapêuticos - Investigação de Resultados.

RESUMEN Introducción: La rotura del ligamento cruzado anterior es una lesión deportiva clínica común y su incidencia tiene una tendencia creciente. El conocimiento por parte de las enfermeras de las mejores prácticas en el postoperatorio de varias cirugías ha colaborado a la pronta vuelta de los pacientes a sus actividades. Urge la necesidad de una optimización en el protocolo hospitalario para este postoperatorio. Objetivo: Optimizar los cuidados de enfermería perioperatorios y los ejercicios funcionales postoperatorios en las lesiones ligamentosas deportivas. Métodos: Se seleccionaron treinta pacientes (18 varones, con una edad de 29,2±8,7 años) con lesión del ligamento cruzado de la rodilla ingresados en el Hospital Universitario de Sanya de enero a diciembre. De tres a cinco días después del ingreso, según el examen físico y químico regular, y la cirugía de reconstrucción del ligamento cruzado bajo anestesia epidural. Se detallaron los cuidados de prevención de las complicaciones postoperatorias, la vigilancia de las constantes vitales y la alternancia de posición en la camilla, las movilizaciones y los cuidados del dolor. La debilidad funcional y el nivel de recuperación causados por la lesión del ligamento de la rodilla se evaluaron según la escala de puntuación de Lysholm y la escala de actividad de Tegner. Resultados: La estancia hospitalaria en este grupo fue de 12 a 43 días, con una media de (25,4±8,6) días. Tras 2 meses de seguimiento, 11 casos mostraron una excelente recuperación funcional, 17 casos de recuperación funcional fueron buenos, el índice de excelente y bueno fue del 93,3%. Tras 6 meses de seguimiento, todos los pacientes de este grupo volvieron al trabajo y a la vida normal. Conclusión: El tratamiento optimizado con entrenamiento funcional en el periodo postoperatorio, los buenos cuidados y los ejercicios adaptados son de gran importancia para la breve recuperación de la función deportiva. Nivel de evidencia II; Estudios terapéuticos - Investigación de resultados.

Effect of beinaglutide treatment on weight loss in Chinese patients with type 2 diabetes mellitus and overweight/obesity

ABSTRACT Objective: To evaluate the effect of beinaglutide on weight loss and plasma protein patterns of inflammation/obesity relevant cytokines and biomarkers. Materials and methods: This study involved 36 adult patients with a body mass index (BMI) of ≥ 24 kg/m2 and T2DM. Beinaglutide was administered for three months. Changes in body weight, fasting plasma glucose (FPG) level, 2 h postprandial plasma glucose (2h-PG) level, glycosylated hemoglobin (HbA1c) level, BMI and visceral and subcutaneous fat areas were measured at baseline and after three months of treatment. In addition, relevant inflammation/obesity cytokines and biomarkers were measured. Results: After three months, beinaglutide treatment led to significant changes, including in body weight, BMI, FPG level, HbA1c level, visceral and subcutaneous fat areas. In addition, serpin E1, leptin, C-reaction protein (CRP) and tumor necrosis factor-α (TNF-α) also decreased significantly. The plasma protein concentrations of CRP (Log2 transformed) were found to be positively correlated with the percentage of weight loss (R = 0.514 and p-value = 0.021). Conclusion: Beinaglutide treatment resulted in weight loss, plasma glucose control and anti-inflammatory effects in patients with T2DM and overweight/obesity.

Knockdown of long non-coding RNA ANRIL inhibits tumorigenesis in human gastric cancer cells via microRNA-99a-mediated down-regulation of BMI1

Long non-coding RNA antisense non-coding RNA in the INK4 locus (ANRIL) has been reported to promote tumorigenesis via regulating microRNA (miR)-99a in gastric cancer cells. However, the role of each component involved in it is still not well understood. This study aimed to verify the role of ANRIL in gastric cancer as well as the underlying mechanisms. ANRIL levels in clinical gastric cancer tissues and cell lines were tested by qPCR. Effects of ANRIL silence on cell viability, migration and invasion, apoptosis, and miR-99a expression in MKN-45 and SGC-7901 cells were measured using CCK-8, Transwell assay, flow cytometry, and qPCR assays, respectively. Then, effects of miR-99a inhibition on ANRIL-silenced cells were evaluated. B-lymphoma Mo-MLV insertion region 1 (BMI1) expression, after abnormal expression of ANRIL and miR-99a, was determined. Finally, expression of key proteins in the apoptotic, Notch, and mTOR pathways was assessed. ANRIL level was elevated in gastric cancer tissues and cell lines. Knockdown of ANRIL suppressed cell viability, migration, and invasion, and increased apoptosis through up-regulating miR-99a. Furthermore, ANRIL silence down-regulated BMI1 via up-regulating miR-99a. BMI1 silence down-regulated Bcl-2 and key kinases in the Notch and mTOR pathways and up-regulated p16 and cleaved caspases. We verified the tumor suppressive effects of ANRIL knockdown in gastric cancer cells via crosstalk with miR-99a. Together, we provided a novel regulatory mechanism for ANRIL in gastric cancer, in which ANRIL silence down-regulated BMI1 via miR-99a, along with activation of the apoptotic pathway and inhibition of the Notch and mTOR pathways.